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Cell Biology
Inhibitors
Protease/Phosphatase Inhibitor Cocktail (100X)
Protease/Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology
Be the first to write a review!
Citations:
(432)
Supplier Page
Supplier Page from
Cell Signaling Technology for
Protease/Phosphatase Inhibitor Cocktail (100X)
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Company Info
Citations
Reviews
Product Specs
Item
Protease/Phosphatase Inhibitor Cocktail (100X)
Company
Cell Signaling Technology
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
5872S
Quantity
1 ml
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Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (432)
(81)
Checkpoint Kinase 1 (CHK1) Functions as Both a Diagnostic Marker and a Regulator of Epithelial-to-Mesenchymal Transition (EMT) in Triple-Negative Breast Cancer
Current issues in molecular biology
November 23, 2022
Kim HJ, Seo BG, Seo EC, Lee KM, Hwangbo C.
1% NP-40 and 1 mM EDTA, protease/phosphatase
inhibitors
cocktail (
Cell signaling
, Danvers, MA, USA,
5872S
)) for 30 min in ice. It was centrifuged at 15,000 rpm at 4 °C for 30 min, and the cell lysate was loaded
(82)
The enhanced association between mutant CHMP2B and spastin is a novel pathological link between frontotemporal dementia and hereditary spastic paraplegias
Acta neuropathologica communications
November 22, 2022
Chen Y, Krishnan G, Parsi S, Pons M, Nikolaki V, Cao L, Xu Z, Gao FB.
lysis buffer (
Thermo Fisher
, Cat. no.
87787
) with protease and phosphatase
inhibitors
(
CST
, catalog no.
5872
). Homogenates were centrifuged at 4 °C for 10 min at 13,000g, to obtain supernatants. Protein concentrations
(83)
NF-κB regulated VentX expression mediates tumoricidal effects of chemotherapeutics at noncytotoxic concentrations
iScience
November 18, 2022
Yi Le, Hong Gao, Angie Zhu, Kristen Felt, Scott Rodig, Ronald Bleday, Zhenglun Zhu
proteins","Chemicals, peptides, and recombinant proteins" ,, Ficoll-Paque plus,
Cell Signaling
Tech.,Cat#
5872
Collagenase D
,
Roche
,Cat#
11088858001
Trizol reagent
,
Life technologies
,Cat#
15596026
Propidium Iodide
(84)
Non-catalytic allostery in α-TAT1 by a phospho-switch drives dynamic microtubule acetylation
The Journal of cell biology
November 7, 2022
Deb Roy A, Gross EG, Pillai GS, Seetharaman S, Etienne-Manneville S, Inoue T.
buffer [#
9806S
;
Cell Signaling Technology
] and mixed with protease/phosphatase
inhibitor
cocktail [#
5872S
;
Cell Signaling Technology
]). Cell lysates were rotated on a wheel at 4°C for 15 min and centrifuged
(85)
Desmin intermediate filaments and tubulin detyrosination stabilize growing microtubules in the cardiomyocyte
Basic research in cardiology
November 3, 2022
Salomon AK, Phyo SA, Okami N, Heffler J, Robison P, Bogush AI, Prosser BL.
EGTA, 10 mM Na3PO4, 0.5 mM GTP, and 1X protease and phosphatase
inhibitor
cocktail (
Cell Signaling
#
5872S
) at pH 6.95.
Microtubule
destabilizing buffer: 0.25 M sucrose, 0.5 mM MgCl2 10 mM Na3PO4, 0.5 mM GTP,
(86)
The Association between Cyclin Dependent Kinase 2 Associated Protein 1 (CDK2AP1) and Molecular Subtypes of Lethal Prostate Cancer
Int J Mol Sci
November 1, 2022
Yaser Gamallat, Andrea Bakker, Ealia Khosh Kish, Muhammad Choudhry, Simon Walker, Saood Aldakheel, Sima Seyedi, Kuo-Cheng Huang, Sunita Ghosh, Geoffrey Gotto,
et al.
(Ref#1861603, Thermo Scientific, USA) containing Protease/Phosphatase Inhibitor Cocktail (
Cell signaling
Cat#
5872S
). Pierce NHS-Activated magnetic beads incubated with 5 μg of
CDK2AP1
monoclonal
mouse
antibody (Santa
(87)
BRAF-V600E utilizes posttranscriptional mechanisms to amplify LPS-induced TNFα production in dendritic cells in a mouse model of Langerhans cell histiocytosis
Journal of leukocyte biology
November 1, 2022
Minichino D, Lv K, Chu N, Tong W, Behrens EM.
no.
78501
;
Thermo Scientific
) in the presence of Protease/Phosphatase Inhibitor Cocktail (catalog no.
5872
;
Cell Signaling Technology
) according to the manufacturer’s instructions, and protein concentrations
(88)
Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis
Nature communications
October 31, 2022
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY.
(10×,
Cell Signaling
, #
9806S
) supplemented with protease
inhibitor
cocktail (100×,
Cell Signaling
, #
5872
). For
immunoprecipitation
, antibodies were coupled to Dynabeads
Protein A
(
Life Technologies
10001D
)
(89)
Coapplication of Magnesium Supplementation and Vibration Modulate Macrophage Polarization to Attenuate Sarcopenic Muscle Atrophy through PI3K/Akt/mTOR Signaling Pathway
International journal of molecular sciences
October 26, 2022
Cui C, Bao Z, Chow SK, Wong RMY, Welch A, Qin L, Cheung WH.
radioimmunoprecipitation assay
(RIPA) buffer with complete mini protease/phosphatase
inhibitor
cocktail (
5872S
,
Cell signaling Technology
, Danvers, MA, USA) according to the manufacturer’s instructions.
Western blot analysis
(90)
A genetically encoded system for oxygen generation in living cells
Proceedings of the National Academy of Sciences
October 25, 2022
Andrew L. Markhard, Jason G. McCoy, Tsz-Leung To, Vamsi K. Mootha
with ice cold 1% Triton lysis buffer supplemented with protease/phosphatase
inhibitor
(
Cell Signaling
#
5872
). Mitochondrial and cytosolic fractions were prepared as previously described (27). Lysates were clarified
(91)
High Intensity Acute Aerobic Exercise Elicits Alterations in Circulating and Skeletal Muscle Tissue Expression of Neuroprotective Exerkines
Brain Plasticity
October 21, 2022
Corey E. Mazo, Edwin R. Miranda, James Shadiow, Michael Vesia, Jacob M. Haus
Cell Signaling Technology
, Danvers, MA) supplemented with 1x Protease/Phosphatase inhibitor cocktail (#
5872
,
Cell Signaling Technology
, Danvers, MA). Protein concentration for each sample was determined by bicinchoninic
(92)
RSPO2 promotes progression of ovarian cancer through dual receptor-mediated FAK/Src signaling activation
iScience
October 21, 2022
Pan R, Yu Y, Zhu H, Zhang W, Qin Y, Ye L, Dai J, Huang R, Peng X, Ye S,
et al
.
10% glycerol) supplemented with Protease/Phosphatase Inhibitor Cocktail (
Cell Signaling Technology
, #
5872
). Lysates were incubated on ice for 20 min and centrifuged at 12,000 rpm for 20 min. Cell lysates were
(93)
Internal checkpoint regulates T cell neoantigen reactivity and susceptibility to PD1 blockade
Med (New York, N.Y.)
October 14, 2022
Palmer DC, Webber BR, Patel Y, Johnson MJ, Kariya CM, Lahr WS, Parkhurst MR, Gartner JJ, Prickett TD, Lowery FJ,
et al
.
(
Cell signaling technology
catalog#
9806
) and protease/phosphatase buffer (
Cell signaling technology
catalog#
5872
) according to the manufacturer’s protocol. Proteins were analyzed using traditional
western blotting
.
(94)
Lysosomal lipid alterations caused by glucocerebrosidase deficiency promote lysosomal dysfunction, chaperone-mediated-autophagy deficiency, and alpha-synuclein pathology
NPJ Parkinson's disease
October 6, 2022
Navarro-Romero A, Fernandez-Gonzalez I, Riera J, Montpeyo M, Albert-Bayo M, Lopez-Royo T, Castillo-Sanchez P, Carnicer-Caceres C, Arranz-Amo JA, Castillo-Ribelles L,
et al
.
(
C5424
) were obtained from
Sigma-Aldrich
(St. Louis, MO, USA).
Protease
/Phosphatase Inhibitor Cocktail (
5872
) was purchased from
Cell Signaling Technology
(Danvers, MA, USA), and the Protease Inhibitor cocktail
(95)
Airway basal cells show a dedifferentiated KRT17(high)Phenotype and promote fibrosis in idiopathic pulmonary fibrosis
Nature communications
September 26, 2022
Jaeger B, Schupp JC, Plappert L, Terwolbeck O, Artysh N, Kayser G, Engelhard P, Adams TS, Zweigerdt R, Kempf H,
et al
.
mM
Tris
(Carl Roth, #4855.2) pH 7.4 in ddH2O with protease/phosphatase
inhibitor
(
Cell Signaling
, #
5872
) and incubated on ice for 20 min. Protein concentrations were determined by BCA Kit (Thermo Fisher Scientific,
(96)
β-Klotho Promotes the Development of Intrauterine Adhesions via the PI3K/AKT Signaling Pathway
International Journal of Molecular Sciences
September 25, 2022
Zizhen Guo, Yuqing Wang, Xiaoyang Wen, Xinxin Xu, Lei Yan
Table 1. 4.5.
Western Blot Analysis
The RIPA lysis buffer containing protease/phosphatase
inhibitor
(
5872
,
Cell Signaling Technology
, Danvers, MA, USA) was used to extract total protein from tissue or cells.
(97)
Inhibiting the activation of MAPK (ERK1/2) in stressed Müller cells prevents photoreceptor degeneration
Theranostics
September 11, 2022
Shaoxue Zeng et al.
buffer with a 1% protease/phosphatase
inhibitor
cocktail (
Cell Signalling
Technology, Danvers, MA,
5872S
), centrifuged at 12,000× g at 4 °C for 10 m and the supernatant was collected. Protein concentration
(98)
Ibrutinib Inhibits Angiogenesis and Tumorigenesis in a BTK-Independent Manner
Pharmaceutics
September 5, 2022
Liu J, Liu Z, Zhang J, Chen X, Chen J, Sui L, Yu J.
Analysis RIPA buffer (#89900; Thermo Fisher Scientific) and Protease/Phosphatase Inhibitor Cocktail (#
5872
;
Cell Signaling Technology
, Danvers, MA, USA) were used to prepare cell lysates at 4 °C. Protein concentrations
(99)
Attenuation of SARS-CoV-2 replication and associated inflammation by concomitant targeting of viral and host cap 2'-O-ribose methyltransferases
The EMBO journal
September 1, 2022
Bergant V, Yamada S, Grass V, Tsukamoto Y, Lavacca T, Krey K, Mühlhofer MT, Wittmann S, Ensser A, Herrmann A,
et al
.
0.1 mM EGTA, 1 mM DTT, and 1 × Protease/phosphatase Inhibitor Cocktail (
Cell Signaling Technology
,
5872S
)). The cell suspension was incubated on ice for 15 min before addition of NP‐40 to the final concentration
(100)
Neuronal NLRP3 is a parkin substrate that drives neurodegeneration in Parkinson's disease
Neuron
August 3, 2022
Panicker N, Kam TI, Wang H, Neifert S, Chou SC, Kumar M, Brahmachari S, Jhaldiyal A, Hinkle JT, Akkentli F,
et al
.
7.4,Quality Biologicals,Cat# 351–086-101 Protease Phosphatase Inhibitor Cocktail,
Cell Signaling
,Cat# #
5872
Paraformaldehyde (PFA) reagent grade,Sigma,Cat# P6148 D.P.X.,Sigma,Cat# 317616 Thionin acetate salt,Sigma,Cat#
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Reviews
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Protease/Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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